a. Background: In this lab, we will be using proteomics to determine the relatedness between species. While a single gene can encode multiple proteins, to what level and where a protein is express or changed such protein after it is translated can result in proteins with very differnent functions. The study of protein structure and functions is called proteomics. The proteome differs from cell to cell and is constantly changing. It is hypothesized that the noncoding DNA controls and regulates protein production. Currently, the study of proteins and their functions are being coordinated by the Human Proteome Organization.
b. Purpose: The purpose of this lab is to compare the proteins of multiple species of fish to determine their relatedness. Proteomics is used to compare other species based on factors beyond their genomes.
c. Process: First we will take multiple species of fish and denature their muscle. By doing so, we will be unraveling the structures of the actin and myosin proteins. Next we will pipet the samples into a protein gel and do gel electrophoresis. After, we will observe the gel results to determine the relatedness between species. The more similar band patterns species have, the more related they are.
d. Hypothesis: I hypothesize that we will find species of fish that are related to one another.
Procedure
Results
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