GTL: Gene Transformation Lab

Introduction
a) Background- In this lab, we will be using a process known as genetic transformation, where we cause a change in an organism by inserting a gene into an organism. Genetic transformation has been used in many sectors of biotechnology. In farming, it has been used to foster resistance to frost, spoilage, and pests. In bioremediation, bacteria can be transformed to digest oil, which is useful in the cleanup of oil spills. In medicine, diseases caused by malfunctioning genes are now being able to be transformed in gene therapy. This could potentially mean that a person's cells that are making them sick could be transformed with healthy copies.
b) Purpose- The purpose of this lab is to show how by inserting a gene into an organism, we can cause a change in that organism. In this instance, we would be transforming a bacteria with a gene that codes for Green Fluorescent Protein that will cause the bacteria to fluoresce under ultraviolet light.
c) Process- First we are labeling one closed test tube +pGLO and another-pGLO. Then we pipet 250 microliters of transformation solution into the tubs and place them on ice. Using a loop, we pick up the +pGLO immerse the solution at the bottom of the tube and then do the same with -pGLO. Then we add 10 microliters of plasmid DNA directly to the liquid in the +pGLO tube and mix the tube. We incubate the tubes on ice for 10 minutes. While the tubes are on ice, we label four agar plates. Then we heat shock the +pGLO and -pGLO tubes. After we open each tube and add 250 microliters of LB nutrient broth to the tubes and close it. We again incubate the tubes for 10 minutes. After we mix the cubes again and pipet 100 microliters of the transformation and control suspensions onto the appropriate plates. Using sterile loops we spread the suspensions evenly on the surfaces of the plates. Final we stack the plates and tape them together and place the stacks upside down in the 37 degrees Celsius incubator for one day. Then we clean up. 
d) Hypothesis- I hypothesize that the transformed bacteria will glow green under ultraviolet light.
Procedure
Results
Discussion